anti onecut2 (Proteintech)

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Proteintech anti onecut2
Anti Onecut2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+onecut2/ONECUT2+Antibody/pmc13002853-94-4-5
Average 93 stars, based on 30 article reviews

anti onecut2 - by Bioz Stars, 2026-09

93/100 stars

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Article Title: Silencing of circRERE(4-5) inhibits ONECUT2-mediated tumorigenesis and metastasis in gastric cancer
Article Snippet: The antibodies used were anti-ONECUT2 (Proteintech, #21916-1-AP; 1:1,000), anti-AGO2 (Proteintech, # 67934-1-Ig; 1:1,000), anti-RERE (abcam, ab217756; 1:1,000), and anti-GAPDH (Proteintech, #10494–1-AP; 1:6,000).

Incubation:

Article Title: Activation of ONECUT2 by RB1 loss in castration-resistant prostate cancer
Article Snippet: Briefly, 100,000 cells from both vector_control and OC2_Overexpression cells were resuspended in wash buffer (20 mM HEPES-NaOH pH 7.5, 150 mM NaCl, 0.5 mM spermidine, and protease inhibitor cocktail), concanavalin A-magnetic beads added, then rotated for 10 min at room temperature. .. Cell-bead conjugates were resuspended in 200 μL of digitonin buffer (wash buffer with 2.5% digitonin solution) containing 2 ug of Anti-Tri-Methyl-Histone H3 (Lys27) (CST, #9733), 2 ug of Anti-Tri-Methyl-Histone H3 (Lys4) (CST, #9751), 5 ug of Anti-ONECUT2 (Proteintech, #21916-1-AP) primary antibody, or rabbit IgG (CST, #66362), rotated overnight at 4°C, resuspended in 250 μL of antibody buffer and 7.5 μL of the pAG-MNase enzyme (#57813, CST), followed by the rotation at 4°C for 1 h. After washing with digitonin buffer, ice-cold 150 μL of digitonin buffer containing CaCl 2 was added and incubated on ice for 30 min followed by the addition of 150 μL of stop buffer containing 5 ng S. cerevisiae spike-in DNA used for sample normalization. ..

Article Title: ONECUT2 acts as a lineage plasticity driver in adenocarcinoma as well as neuroendocrine variants of prostate cancer.
Article Snippet: .. Cell-bead conjugates were resuspended in 200 μl of digitonin buffer (wash buffer with 2.5% digitonin solution) containing 2ug of AntiTri-Methyl-Histone H3 (Lys27) (CST, #9733), 2ug of AntiTri-Methyl-Histone H3 (Lys4) (CST, #9751), 2ug of AntiAcetyl-Histone H3 (Lys27) (D5E4) (CST, #8173), 2ug of AntiMono-Methyl-Histone H3 (Lys4) (D1A9) (CST, #5326), 5 ug of Anti-ONECUT2 (Proteintech, #21916-1-AP), 5ug of AntiGR (D6H2L) (CST, #12041), 5ug of Anti-AR (Activemotif, #39781), 5 ug of Anti-FOXA1 (Activemotif, #398837) primary antibody, or rabbit IgG (CST, # 66362,), rotated overnight at 4 ◦C, resuspended in 250 μl of antibody buffer and 7.5 μl of the pAG-MNase enzyme (# 57813, CST), followed by the rotation at 4 ◦C for 1 h. After washing with digitonin buffer, ice-cold 150 μl of digitonin buffer containing CaCl2 was added and incubated on ice for 30 min followed by the addition of 150 μl of stop buffer containing 5 ng S. cerevisiae spike-in DNA used for sample normalization. ..

Article Title: ONECUT2 acts as a lineage plasticity driver in adenocarcinoma as well as neuroendocrine variants of prostate cancer
Article Snippet: .. Cell-bead conjugates were resuspended in 200 μl of digitonin buffer (wash buffer with 2.5% digitonin solution) containing 2ug of Anti-Tri-Methyl-Histone H3 (Lys27) (CST, #9733), 2ug of Anti-Tri-Methyl-Histone H3 (Lys4) (CST, #9751), 2ug of Anti-Acetyl-Histone H3 (Lys27) (D5E4) (CST, #8173), 2ug of Anti-Mono-Methyl-Histone H3 (Lys4) (D1A9) (CST, #5326), 5 ug of Anti-ONECUT2 (Proteintech, #21916-1-AP), 5ug of Anti-GR (D6H2L) (CST, #12041), 5ug of Anti-AR (Activemotif, #39781), 5 ug of Anti-FOXA1 (Activemotif, #398837) primary antibody, or rabbit IgG (CST, # 66362,), rotated overnight at 4 °C, resuspended in 250 μl of antibody buffer and 7.5 μl of the pAG-MNase enzyme (# 57813, CST), followed by the rotation at 4 °C for 1 h. After washing with digitonin buffer, ice-cold 150 μl of digitonin buffer containing CaCl2 was added and incubated on ice for 30 min followed by the addition of 150 μl of stop buffer containing 5 ng S. cerevisiae spike-in DNA used for sample normalization. ..

Article Title: ONECUT2 Activates Diverse Resistance Drivers of Androgen Receptor-Independent Heterogeneity in Prostate Cancer
Article Snippet: .. Cell-bead conjugates were resuspended in 200 μL of digitonin buffer (wash buffer with 2.5% digitonin solution) containing 2ug of Anti-Tri-Methyl-Histone H3 (Lys27) (CST, #9733), 2ug of Anti-Tri-Methyl-Histone H3 (Lys4) (CST, #9751), 2ug of Anti-Acetyl-Histone H3 (Lys27) (D5E4) (CST, #8173), 2ug of Anti-Mono-Methyl-Histone H3 (Lys4) (D1A9) (CST, #5326), 5ug of Anti-ONECUT2 (Proteintech, #21916–1-AP), 5ug of Anti-Glucocorticoid Receptor (D6H2L) (CST, #12041), 5ug of Anti-AR (Activemotif, #39781), 5ug of Anti-FOXA1 (Activemotif, #398837) primary antibody, or rabbit IgG (CST, # 66362,), rotated overnight at 4 °C, resuspended in 250 μL of antibody buffer and 7.5 μL of the pAG-MNase enzyme (# 57813, CST), followed by the rotation at 4 °C for 1 h. After washing with digitonin buffer, ice-cold 150 μL of digitonin buffer containing CaCl2 was added and incubated on ice for 30 min followed by the addition of 150 μL of stop buffer containing 5 ng S. cerevisiae spike-in DNA used for sample normalization. ..

Article Title: ONECUT2 Activates Diverse Resistance Drivers of Androgen Receptor-Independent Heterogeneity in Prostate Cancer
Article Snippet: .. Cell-bead conjugates were resuspended in 200 μL of digitonin buffer (wash buffer with 2.5% digitonin solution) containing 2ug of Anti-Tri-Methyl-Histone H3 (Lys27) (CST, #9733), 2ug of Anti-Tri-Methyl-Histone H3 (Lys4) (CST, #9751), 2ug of Anti-Acetyl-Histone H3 (Lys27) (D5E4) (CST, #8173), 2ug of Anti-Mono-Methyl-Histone H3 (Lys4) (D1A9) (CST, #5326), 5ug of Anti-ONECUT2 (Proteintech, #21916-1-AP), 5ug of Anti-Glucocorticoid Receptor (D6H2L) (CST, #12041), 5ug of Anti-AR (Activemotif, #39781), 5ug of Anti-FOXA1 (Activemotif, #398837) primary antibody, or rabbit IgG (CST, # 66362,), rotated overnight at 4 °C, resuspended in 250 μL of antibody buffer and 7.5 μL of the pAG-MNase enzyme (# 57813, CST), followed by the rotation at 4 °C for 1 h. After washing with digitonin buffer, ice-cold 150 μL of digitonin buffer containing CaCl2 was added and incubated on ice for 30 min followed by the addition of 150 μL of stop buffer containing 5 ng S. cerevisiae spike-in DNA used for sample normalization. ..

ChIP-qPCR:

Article Title: Transcriptional landscape and chromatin accessibility reveal key regulators for liver regenerative initiation and organoid formation.
Article Snippet: Article

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